描述

Thermo Scientific SILAC Protein Quantitation Kit - DMEM is used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

Features of SILAC Protein Quantitation Kit - DMEM:

• Efficient—100% label incorporation into proteins of living cells
• Reproducible—eliminates intra-experimental variability caused by differential sample preparation
• Flexible—media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling
• Compatible—label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others
• High-quality supplements—heavy amino acids with >99% isotope purity; dialyzed FBS tested to ensure that it is sterile, endotoxin-free, and cell culture compatible

Applications of SILAC Protein Quantitation Kit:
• Quantitative analysis of relative changes in protein abundance from different cell treatments
• Quantitative analysis of proteins for which there are no antibodies available
• Protein expression profiling of normal vs. disease cells
• Identification and quantification of hundreds to thousands of proteins in a single experiment

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails thein vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

规格

内容及储存

Upon receipt store media at 4°C, Dialyzed Fetal Bovine Serum (FBS) at -20°C and amino acids at room temperature. The Dialyzed FBS is shipped separately with dry ice; all other components are shipped at ambient temperature.