1-step human couple IVT Kit

描述

Thermo Scientific 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100 µg/mL per reaction.

The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitrotranslation or coupled transcription/translation reactions. Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100 µg/mL when combined with the optimized pT7CFE1 Expression Vector. The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity.

Features of the 1-Step Human Coupled IVT Kit:

• Functional—uses the human translational machinery to express active proteins
• Convenient—perform transcription and translation in a single step
• High performance—greater yields compared to rabbit reticulocyte in vitro translation
• Reliable—express proteins that fail in rabbit reticulocyte systems

Better Than Traditional Methods:
• HeLa cell-free extracts are capable of expressing proteins with post-translational modifications
• Accurate translation delivers full-length protein compatible with downstream applications
• Protein translation is optimized with EMCV IRES element and other mRNA stabilizing elements

规格

内容及储存

Sufficient For: 40 reactions of 25 µL each
• HeLa Lysate, 500 µL
• Accessory Proteins, 100 µL
• Reaction Mix, 200 µL
• Positive Control DNA: pCFE-GFP, 10 µg
• pT7CFE1-CHis, 10 µg
• Nuclease-Free Water, 1.5 mL

Upon receipt store kit at -80°C. Product is shipped on dry ice