Anti-GFP抗体- ChIP Grade (ab290)

产品名称

Anti-GFP抗体- ChIP Grade

参阅全部 GFP 一抗

描述

兔多克隆抗体to GFP - ChIP Grade

宿主

Rabbit

特异性

Anti-GFP antibody - ChIP Grade (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

This anti-GFP antibody recognizes the enhanced form of GFP as well.

Recognises EYFP (Yellow Fluorescent Protein (YFP), a genetic mutant of green fluorescent protein (GFP).

ab290 also detects venus YFP.

经测试应用

适用于: ELISA, ICC/IF, ChIP, IHC-FrFl, Electron Microscopy, IHC-FoFr, ICC, IHC-P, IHC-Fr, IP, WBmore details

种属反应性

与反应: Species independent

免疫原

Recombinant full length protein corresponding to GFP.

Database link: P42212

阳性对照

Detects 5ng of recombinant GFP (using ECL or ECL Plus) in under one minute of exposure to film. Pure GFP protein, or cells known to overexpress GFP.

常规说明

The total IgG concentration has been determined to be 5 mg/mL. The specific IgG concentration is unknown. This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination. 

Anti-GFP antibody (ab6556) is the purified version of this antibody (see Related Products).