Superdex 200 Increase 10/300 GL

Superdex 200 Increase 10/300 GL is a versatile, prepacked column for size exclusion chromatography in small-scale (mg) preparative purification as well as for characterization and analysis of proteins with molecular weights between 10 000 and 600 000, such as antibodies.

• Increased resolution vs Superdex 200.

• Reduced runtime vs Superdex 200 for quicker results.

• Versatile use for both preparative and analytical purposes.

• Suitable for a broad range of proteins, especially for antibodies

Superdex 200 Increase is the next generation of size exclusion chromatography (gel filtration) resins offering versatility and higher resolution within a shorter time frame compared to Superdex 200.

The Superdex 200 Increase 10/300 GL column is well suited for small-scale (25 – 500 µL) preparative purification such as the polishing step in a purification procedure, where mg amounts of highly size-homogenous target proteins can be achieved. It is also well suited for characterization of proteins, such as aggregate analysis of antibodies, size homogeneity analysis of membrane proteins, or protein-protein interactions, where high-quality data can be achieved.

Smaller Bead Size Increases Resolution

The increased resolution of Superdex 200 Increase compared to Superdex 200 is a result of the smaller bead size (mean bead size 8.6 µm), giving higher purity and better analysis results.

The Superdex 200 Increase 10/300 GL column is a versatile tool in your protein purification toolbox, perfectly suited for e.g., antibodies. The resin has a broad fractionation range that allows separation of a large variety of proteins (M_r 10 000 to 600 000) with an optimized resolution for the antibody molecular weight range (M_r 100 000 to 300 000).

Clean-In-Place for Versatility

Superdex 200 Increase resin is alkali resistant and supports clean-in-place (CIP) procedures, which allows you to benefit from the column versatility by using the same column for different proteins. Alkali resistance differentiates agarose-based resins from silica-based resins. In addition, the possibility to the clean the resin ensures a long lifetime forthe column.

What People Are Saying

Superdex 200 Increase reduces your runtime compared to Superdex 200. The high-flow agarose base matrix has excellent pressure/flow properties, which in combination with the smaller bead size allows for high flow rates and increased resolution.

Consumables

Select to expand the desired collection of consumables.

For consumables not listed in this section, please contact your local GE Healthcare service representative.

Superdex 200 Increase 10/300 GL

Bed Volume	Approximately 24 ml
Complete Packsize	1
Sample Volume	25 µl – 500 µl
Flow Rate	< 1.80 ml/min1)
Storage	4° to 30° C, 20% ethanol
Storage Conditions	10 ROOM TEMPERATURE
Pressure Max. [Over the Packed Bed During Operation]	< 3.0 MPa [30 bar] (435 psi)
Recommended Flow Rate	0.75 ml/min
Theoretical Plates	> 48 000 m-1
1)Water at room temperature

Media
Matrix	Composite of cross-linked agarose and dextran
pH Stability Working Range	3–12
Storage Conditions	4° to 30° C, 20% ethanol
pH stability, CIP	1–14
Exclusion Limit [Mr] [Globular Proteins]	Approx 1.3 x 106
Chemical Stability	Long term use: Long term use refers to use where the medium is stable over a long period of time without adverse side effects on its chromatographic performance. • All commonly used aqueous buffers, pH 3 to 12 • Urea, up to 8 M • Ionic and non-ionic detergents • Guanidine hydrochloride, up to 6 M Short term use: Short term use refers to the use during regeneration, cleaning-inplace, and sanitization procedures. • Acetonitrile, up to 30% • Sodium hydroxide, up to 1 M • Ethanol, up to 70% • Methanol, up to 100% • Acetic acid, up to 1 M • Isopropanol, up to 30% • Hydrochloric acid, up to 0.1 M • Trifluoroacetic acid, up to 10% • Formic acid, up to 70% Avoid: • Oxidizing agents • Unfiltered solutions

Column
Column i.d.	10 mm
Bed Height With One Adaptor	279 mm-309 mm
Bed Height With Two Adaptors	245 mm-306 mm
Complete Packsize	1 Piece
Chemical Stability	Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons
Material [Column Hardware]	Borosilicate Glass, Polyetheretherketone (PEEK), Polypropylene (PP), Ethylene Propylene Diene Monomer (EPDM) and Perfluoro-rubber (PFR)
Material [Column Tube]	Borosilicate Glass
Material [Bed Support]	Polyethylene (PE) filter
Bed Support	Filter
Porosity	7 μm