BV421 Mouse Anti-Human CD3 

Clone  SP34-2   (RUO)

BrandBD Horizon™

Alternative NameCD3E; CD3-epsilon; T3E; TCRE

Vol. Per Test5 µl

IsotypeMouse BALB/c IgG1, κ

ReactivityRhesus, Cynomolgus, Baboon (QC Testing)  Human (Tested in Development) 

ApplicationFlow cytometry (Routinely Tested) 

ImmunogenPurified Human CD3ε Protein

Storage BufferAqueous buffered solution containing BSA and ≤0.09% sodium azide.

Description

Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cells markers. SP34-2 is also capable of inducing cell proliferation on both human and non-human primate PBMC.The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

FORMAT

Format

• BV421

• Excitation SourceViolet 405 nm

• Excitation Max407 nm

• Emission Max421 nm

BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.