BD PE-Cy™7 Mouse Anti-Human TNF  Clone  MAb11   (RUO) 560678

PE-Cy™7 Mouse Anti-Human TNF 

Clone  MAb11   (RUO)

BrandBD Pharmingen™

Alternative NameTumor necrosis factor alpha; TNF-a; TNF-α; TNFSF2; Cachectin

Vol. Per Test5 µl

IsotypeMouse IgG1, κ

ReactivityRhesus, Baboon, Cynomolgus (QC Testing)  Human (Tested in Development) 

ApplicationIntracellular staining (flow cytometry) (Routinely Tested) 

ImmunogenRecombinant Human TNF

Storage BufferAqueous buffered solution containing BSA and ≤0.09% sodium azide.

Description

The MAb11 monoclonal antibody specifically binds to human tumor necrosis factor (TNF, also known as TNF-α) protein. TNF is an efficient juxtacrine, paracrine and endocrine mediator of inflammatory and immune functions. It regulates the growth and differentiation of a variety of cell types. TNF is cytotoxic for transformed cells when in conjunction with IFN-γ. It is secreted by activated monocytes/macrophages and other cells such as B cells, T cells and fibroblasts. The immunogen used to generate the MAb11 hybridoma was recombinant human TNF. The MAb11 antibody has been reported to crossreact with Rhesus Macaque TNF.MAb11 has been predicted to be reactive on other non-human primate samples (e.g Baboon, Cynomolgus). Investigators are advised that the PE-Cy™7 Mouse Anti-Human TNF (clone MAb11) antibody is not routinely tested on these other non-human primate samples.

FORMAT

• FormatPE-Cy™7

• Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm

• Excitation Max496 nm

• Emission Max785 nm

PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.