More Information

Product Details

Synonyms Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase

Product Type Monoclonal Antibody

Properties

Clone OMNI379

Isotype Mouse IgG2aκ

Immunogen/Antigen Recombinant human Nampt (visfatin/PBEF).

Application

ELISA: (direct or indirect: 1:2'000-1:10'000)

Flow Cytometry: (1:1'000)

Immunocytochemistry: (1:500-1:1’000)

Immunohistochemistry: (paraffin sections, 1:100-1:500)

Immunoprecipitation: (1:500-1:1’000)

Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).

Optimal conditions should be determined individually for each application.

Crossreactivity Human

Mouse

Rat

Specificity

Recognizes human, mouse and rat Nampt.

Purity Detail Protein G-affinity purified.

Concentration 1mg/ml

Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Isotype Negative Control

Mouse IgG2a Isotype Control

Shipping and Handling

Shipping BLUE ICE

Short Term Storage +4°C

Long Term Storage -20°C

Handling Advice After opening, prepare aliquots and store at -20°C.

Avoid freeze/thaw cycles.

Use/Stability Stable for at least 1 year after receipt when stored at -20°C.