BioMed 常用的发色底物 
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BIOPHENTM Factor VIIa

REF 221312

R1 R3 2 x 4 mL, R2 2 x 2 mL, R4 2 x 25 mL

Quantitative determination of Factor VIIa activity, in purified medium, using chromogenic assay.

FOR RESEARCH USE ONLY.

DO NOT USE IN DIAGNOSTIC PROCEDURES.

INTENDED USE:

The BIOPHEN™ Factor VIIa kit is a chromogenic method intended for in vitro quantitative determination of activated Factor VII (FVIIa) activity, in purified milieu, using manual or automated method.

This kit is for research use only and must not be used for patient diagnosis or treatment.

PRINCIPLE:

Factor VII is the serine esterase of the extrinsic coagulation pathway. When complexed to Tissue Factor (TF), in presence of phospholipids and calcium, it activates Factor X to Factor Xa.

The BIOPHEN™ Factor VIIa kit is insensitive to Factor VII.

Factor VIIa forms an enzymatic complex with recombinant truncated human Tissue Factor (rTTF) and synthetic phospholipids. It then activates Factor X, present in the assay at a constant concentration and in excess, to Factor Xa. The FXa activity is measured using a specific chromogenic substrate (SXa-11). Factor Xa cleaves the substrate and generates pNA. The amount of pNA generated is directly proportional to the Factor Xa activity. Finally, there is a direct relationship between the amount of Factor VIIa in the assayed sample and the Factor Xa activity generated, measured by the amount of pNA released, determined by colour development at 405 nm.