描述

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time. 

• Contains AmpliTaq Gold DNA Polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq DNA Polymerase 
• Includes a passive internal reference based on proprietary ROX dye, for superb precision on Applied Biosystems real-time PCR instruments 
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences 
• AmpErase UNG protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination 
• Rapid assay development guidelines are provided to minimize optimization time

规格

Detection Method:Primer-Probe Detection
For Use With (Equipment):7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Reaction Speed:Standard
PCR Method:qPCR
Sample Type (General):DNA
Passive Reference Dye:ROX (Pre-mixed)
Polymerase:AmpliTaq Gold DNA Polymerase
Primer-Probe Compatibility:TaqMan®
Product Line:TaqMan™
Product Size:1 x 5 mL
Sample Type (Specific):DNA (Genomic), cDNA

内容及储存

Supplied at 2X concentration. The mix contains AmpliTaq Gold® DNA Polymerase, Uracil-DNA Glycosylase, dNTPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains one 5 mL tube, sufficient for 200 reactions at the 50 uL total volume. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).