TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.
• Contains AmpliTaq Gold DNA Polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq DNA Polymerase
• Includes a passive internal reference based on proprietary ROX dye, for superb precision on Applied Biosystems real-time PCR instruments
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• AmpErase UNG protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination
• Rapid assay development guidelines are provided to minimize optimization time
Detection Method: | Primer-Probe Detection |
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For Use With (Equipment): | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Reaction Speed: | Standard |
PCR Method: | qPCR |
Sample Type (General): | DNA |
Passive Reference Dye: | ROX (Pre-mixed) |
Polymerase: | AmpliTaq Gold DNA Polymerase |
Primer-Probe Compatibility: | TaqMan® |
Product Line: | TaqMan™ |
Product Size: | 1 x 5 mL |
Sample Type (Specific): | DNA (Genomic), cDNA |
Supplied at 2X concentration. The mix contains AmpliTaq Gold® DNA Polymerase, Uracil-DNA Glycosylase, dNTPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains one 5 mL tube, sufficient for 200 reactions at the 50 uL total volume. Store at 2-8°C
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).