HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.
HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.
Convenient desalting and buffer exchange of sample volumes up to 1.5 mL.
Two to five columns can be connected in series for sample sizes from 3 mL to 7.5 mL.
Replaces dialysis for desalting protein, which saves time and reduces labor.
Requires less than one minute with typically > 95% recovery.
HiTrap column format is convenient for use with a syringe, a peristaltic pump, or a chromatography system.
HiTrap Desalting is a prepacked, ready-to-use column for group separation between high and low molecular weight substances. Applications include buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants, or removal of reagents to terminate a reaction.
Parameter | HiTrap Desalting, 100 x 5 ml |
---|---|
Bed dimensions | 16 × 25 mm |
Bed height | 25 mm |
Bed volume | 5 |
Column i.d. | 16 mm |
Flow rate1 | < 15 ml/min |
Order Information | Pack size available by special order. |
Pressure max. (over the packed bed during operation) | 5 bar [0.5 MPa] (70 psi) |
Sample volume | <= 1.5 ml |
Storage | 4 to 30°C, 20% Ethanol |
Pack size | 100 x 5 mL |