HiTrap Desalting columns with Sephadex G-25 resin

HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.

HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.

  • Convenient desalting and buffer exchange of sample volumes up to 1.5 mL.

  • Two to five columns can be connected in series for sample sizes from 3 mL to 7.5 mL.

  • Replaces dialysis for desalting protein, which saves time and reduces labor.

  • Requires less than one minute with typically > 95% recovery.

  • HiTrap column format is convenient for use with a syringe, a peristaltic pump, or a chromatography system.

Column for desalting and buffer exchange of proteins

HiTrap Desalting is a prepacked, ready-to-use column for group separation between high and low molecular weight substances. Applications include buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants, or removal of reagents to terminate a reaction.

ParameterHiTrap Desalting, 100 x 5 ml
Bed dimensions16 × 25 mm 
Bed height25 mm 
Bed volume
Column i.d.16 mm
Flow rate1< 15 ml/min 
Order InformationPack size available by special order. 
Pressure max. (over the packed bed during operation)5 bar [0.5 MPa] (70 psi) 
Sample volume<= 1.5 ml 
Storage4 to 30°C, 20% Ethanol 
Pack size100 x 5 mL