LightCycler® Multiplex RNA Virus Master

One-step RT-qPCR Master Mix for sensitive and reliable multiplex detection of viral RNA targets and internal controls.
For general laboratory use.
Overview:
The combination of a unique reverse transcriptase enzyme solution and the aptamer-mediated hot start AptaTaq Fast DNA Polymerase, guarantees fast and highly specific one-step RT-qPCR of viral RNA targets isolated from a variety of human sample materials.

• Unique recombinant reverse transcriptase for efficient and robust reverse transcription of RNA isolated from a wide range of materials, including difficult sample materials (e.g., blood, plasma, serum, and stool).

• Highly sensitive reverse transcription and multiplex qPCR detection.

• Fast results and specific priming due to an aptamer-mediated hot start protocol.

• Especially suited for automated workflows due to high thermostability of reverse transcriptase and AptaTaq Fast DNA Polymerase.

• Contents:

1. RT-Enzyme Solution, 200x conc.

2. RT-PCR Reaction Mix, 5x conc.

3. Water, PCR grade

Application:
The LightCycler® Multiplex RNA Virus Master consists of a unique recombinant reverse transcriptase (200x concentrated) and a complete 5x PCR reaction mix with AptaTaq Fast DNA Polymerase, dNTPs, and a special reaction mix for multiplex qPCR. AptaTaq Fast DNA Polymerase is a thermostable, recombinant Taq polymerase with aptamer-mediated, reversible hot start. It is immediately activated by heating, which allows specific priming and fast PCR.