anti-Caspase-1 (p20) (mouse), mAb (Casper-1)

[Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE]

AG-20B-0042-C100 100 μg

Clone Casper-1

Source/Host Purified from concentrated hybridoma tissue culture supernatant.

Isotype Mouse IgG1

Immunogen Recombinant mouse caspase-1.

Handling / Storage

Shipping BLUE ICE

Short Term Storage +4°C

Long Term Storage -20°C

After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Use / Stability

Stable for at least 1 year after receipt when stored at -20°C.

MSDS available at www.adipogen.com or upon request.

Product Specifications

Specificity Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1.

Species Crossreactivity Mouse

Application Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant

for the detection of caspase-1 (mouse) upon inflammasome activation)

Immunohistochemistry: (1:500; paraffin sections)

Immunoprecipitation: (1:200)

Purity ≥95% (SDS-PAGE)

Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.

Concentration 1mg/ml

Product Description

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and

IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes

in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic

flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds

to a variety of agonists including crystals.

Figure 1: Mouse caspase-1 (p20) is detected by immunoblotting using anti-Caspase-1 (p20) (mouse), mAb

(Casper-1) (Prod. No. AG-20B-0042). Method: Caspase-1 was analyzed by Western blot in cell extracts and

supernatants of differentiated bone marrow-derived dendritic cells (BMDCs) from wild-type, NLRP3-/- and caspase-

1-/- mice activated or not by 5 μM Nigericin (Prod. No. AG-CN2-0020) for 30 min. Cell extracts and supernatants

were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-

Caspase-1 (p20) (mouse), mAb (Casper-1) (1μg/ml). Proteins were visualized by a chemiluminescence detection

system.