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商品详情

Description
OverviewSerine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
Catalogue Number539480
Brand FamilyCalbiochem®

References
ReferencesWatazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.
Product Information
CAS number39450-01-6
Activity≥30 mAnsonU/mg dry weight
Unit of DefinitionOne mAnson unit is defined as the amount of enzyme that will liberate 1.0 µmol of Folin-positive amino acids (calculated as tyrosine) per min at 25°C, pH 7.5.
EC number3.4.21.14
FormWhite lyophilized solid
FormulationLyophilized from calcium acetate

Biological Information
Specific Activity≥40 mAnsonU/mg protein

Physicochemical Information
ContaminantsDNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
Storage and Shipping Information
Ship CodeAmbient Temperature Only
ToxicityIrritant
Storage+2°C to +8°C
Do not freezeOk to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.


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