描述

Thermo Scientific HisPur Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Features of HisPur Ni-NTA Resin:

• High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
• Versatile—purify proteins using native or denaturing conditions
• Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
• Cost-effective—reuse the same batch of resin at least five times
• Easy to use—pre-formulated buffers available for kit formats
• Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

规格

内容及储存

Store at 4°C