磁珠用于标记和分离细胞或其他生物质，如抗体或配合基.....。

描述：

Anti-Biotin MicroBeads have been developed for indirect magnetic labeling and separation of cells or other biological materials that are labeled with a biotinylated primary antibody or ligand, or with a cocktail of biotinylated antibodies. When working with debris-rich starting material and low frequency samples we recommend Anti-Biotin MicroBeads UltraPure to benefit from high specific enrichment with low background of debris and dead cells.

1. Description Components 2 mL Anti-Biotin MicroBeads: MicroBeads conjugated to monoclonal mouse anti-biotin antibodies (isotype: mouse IgG1). Capacity For 10⁹ total cells, up to 100 separations. Product format Anti-Biotin MicroBeads are supplied in buffer containing stabilizer and 0.05% sodium azide. Storage Store protected from light at 2−8 °C. Do not freeze. The expiration date is indicated on the vial label. 1.1 Principle of the MACS® Separation First, the biological material of interest (e.g. cells, bacteria, or subcellular material) is labeled with biotinylated antibodies or ligands. Subsequently, the material is magnetically labeled with Anti-Biotin MicroBeads. Then, the cell suspension is loaded onto a MACS® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled material is retained within the column. The unlabeled material runs through; this cell fraction is thus depleted of magnetically labeled material. After removing the column from the magnetic field, the magnetically retained material can be eluted as the positively selected cell fraction. To increase the purity, the positively selected cell fraction containing the magnetically labeled cells can be separated over a second column. 1.2 Background information Anti-Biotin MicroBeads have been developed for indirect magnetic labeling and separation of cells or other biological materials that are labeled with a biotinylated primary antibody or ligand, or with a cocktail of biotinylated antibodies. The biotinylated molecule is recognized by a monoclonal anti-biotin antibody coupled to MicroBeads. Anti-Biotin MicroBeads have the advantage of not binding to free biotin, which is often present in culture media. This feature provides the most sensitive labeling and separation of cells

注意事项：

Reagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, which is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive conditions may develop.