Streptavidin Sepharose High Performance, 5 ml

Streptavidin Sepharose High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules.

  • Streptavidin immobilized to Sepharose High Performance for purification of biotinylated compounds

  • Extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.

  • 34 µm bead size for improved resolution


Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose High Performance. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilised streptavidin binds biotin and biotinylated substances through affinity chromatography.

Particle size, d50V34 µm1)
MatrixHighly cross-linked agarose, 6%
Binding Capacity/ml Chromatography Medium>300 nmol biotin/ml medium
6 mg biotinylated BSA/ml medium
pH Stability Working Range4–9
pH stability, CIP2–10.5
Flow Velocity<150 cm/h
Storage Conditions4 to 8°C, 20% Ethanol
1)d50v is the median particle size of the cumulative volume distribution.