Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
With |
• L-glutamine |
• HEPES |
• Phenol Red |
The complete formulation is available.
Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified
Glutamine: | L-Glutamine |
---|---|
Phenol Red Indicator: | Phenol Red |
Form: | Liquid |
HEPES Buffer: | HEPES |
Serum Supplementation: | Standard Serum Supplementation |
Sodium Pyruvate Additive: | No Sodium Pyruvate |
Product Size: | 500 mL |
Volume (Metric): | 500 ml |
Shipping Condition: | Room Temperature |
22400 - RPMI 1640, HEPES