描述

Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of  Gibco® trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature:

• Quality testing
• Documented traceability
• Dual-site cGMP Manufacturing

This trypsin solution is modified as follows:

With
• EDTA
• Phenol Red


The complete formulation is available.

Quality testing
Gibco® Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP manufacturing and quality system
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

规格

Chelators:EDTA
Phenol Red Indicator:Phenol Red
Classification:Animal Origin
Form:Liquid
pH Range:7.2 - 8.0
Osmolality:270 - 310 mOsm/kg
Concentrated:1 X
Product Size:100 mL
Reagent Type:Trypsin
Tests Performed:In Vitro Bioassay
Shipping Condition:Wet Ice

内容及储存

Storage conditions: -5°C to -20°C
Shelf life: 24 months from date of manufacture

培养基配方

25300 - 0.05% Trypsin - EDTA (1x), phenol red