描述

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. Life Technologies offers a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

With
• L-glutamine
• Phenol Red


The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco® F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.

规格

Form:Liquid
Glutamine:L-Glutamine
HEPES Buffer:No HEPES
Product Size:500 mL
Volume (Metric):500 ml
Phenol Red Indicator:Phenol Red
Sodium Pyruvate Additive:Sodium Pyruvate
Shipping Condition:Room Temperature

培养基配方

11765 - Ham's F-12 Nutrient Mix