描述

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This M199 is modified as follows:

WithWithout
• L-glutamine• HEPES
• Phenol Red• Sodium bicarbonate


The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hank's salts for use without CO2.

In Vitro Diagnostic
Gibco® Medium 199 is manufactured in compliance with the requirements of ISO 13485: 2003 for the design, development, manufacture, and distribution of liquid and powder tissue culture media, sera, and reagents for cell culture research, industrial bioprocessing, and related markets.

Dual-Site cGMP Manufacturing
Gibco® Medium 199 is manufactured in a cGMP New York facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001. For supply chain continuity, Life Technologies offers a comparable Gibco® Medium 199 product made in the Scotland facility (31100-027). This second cGMP manufacturing location is also registered with the FDA as a medical device manufacturer and is certified to ISO 13485. Medium 199 

规格

Glutamine:L-Glutamine
Phenol Red Indicator:Phenol Red
Form:Powder
HEPES Buffer:No HEPES
Sodium Pyruvate Additive:No Sodium Pyruvate
Glucose:Low Glucose
Product Size:10 x 1 L
Shipping Condition:Room Temperature

培养基配方

31100 - Medium 199, powder